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Dunn, JA thesis.pdf (1.83 MB)
ETD Abstract Container
Abstract Header
Single Molecule Characterization of Peptide/Hematite Binding
Author Info
Dunn, James Albert
ORCID® Identifier
http://orcid.org/0000-0003-0940-8232
Permalink:
http://rave.ohiolink.edu/etdc/view?acc_num=osu1494014864020062
Abstract Details
Year and Degree
2017, Master of Science, Ohio State University, Environment and Natural Resources.
Abstract
This study utilized atomic force microscopy (AFM) to study single-molecule interactions between hematite-binding peptides and a hematite-coated surface. The goal of this study was to investigate the relationship between amino acid sequence and peptide affinity for hematite (α-Fe2O3), with a focus on the influence of serine versus threonine residues in a peptide sequence. Data from these single molecule binding experiments suggest that substituting serine with threonine in a hematite-binding peptide increases peptide affinity for a hematite surface. Molecular dynamics (MD) simulations, carried out by our collaborators, were done in parallel with the single molecule binding experiments to model how two serine to threonine substitutions would alter how peptides bound to a hematite matrix. The results of these MD simulations agree with the trends from our single molecule experiments and support the conclusion that replacing serine residues with threonine residues increases peptide affinity for hematite. These findings could be utilized to improve existing biotechnology, including biological fuel cells, biosensing, solar energy production, and medical treatment in humans.
Committee
Brian Lower, PhD (Advisor)
Steven Lower, PhD (Committee Member)
Michael Wilkins, PhD (Committee Member)
Deric Learman, PhD (Committee Member)
Pages
53 p.
Subject Headings
Biophysics
Keywords
force spectroscopy
;
surface functionalization
;
peptides
;
hematite
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Citations
Dunn, J. A. (2017).
Single Molecule Characterization of Peptide/Hematite Binding
[Master's thesis, Ohio State University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=osu1494014864020062
APA Style (7th edition)
Dunn, James.
Single Molecule Characterization of Peptide/Hematite Binding.
2017. Ohio State University, Master's thesis.
OhioLINK Electronic Theses and Dissertations Center
, http://rave.ohiolink.edu/etdc/view?acc_num=osu1494014864020062.
MLA Style (8th edition)
Dunn, James. "Single Molecule Characterization of Peptide/Hematite Binding." Master's thesis, Ohio State University, 2017. http://rave.ohiolink.edu/etdc/view?acc_num=osu1494014864020062
Chicago Manual of Style (17th edition)
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Document number:
osu1494014864020062
Download Count:
85
Copyright Info
© 2017, all rights reserved.
This open access ETD is published by The Ohio State University and OhioLINK.