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Brawnie Petrov Masters Thesis .pdf (1.05 MB)

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A New Role for Vitamin D Binding Protein in Bipolar Disorder

Petrov, Brawnie Rebecca
http://orcid.org/0000-0002-2272-5884
http://rave.ohiolink.edu/etdc/view?acc_num=osu1492642404941773

Abstract Details

2017, Master of Science, Ohio State University, Human Ecology: Human Nutrition.
Genetic, dietary and inflammatory factors contribute to the etiology of major mood disorders (MMD), thus impeding the identification of specific biomarkers to assist in diagnosis and treatment. We tested the association of vitamin D and inflammatory markers in 36 adolescents with bipolar disorder (BD) and major depressive disorder (MDD) forms of MMD and without MMD (non-mood control). We also assessed the overall level of inflammation using a cell-based reporter assay for nuclear factor-kappa B activation and measuring antibodies to oxidized LDL. We found that these factors were similar between non-mood and MMD youth. To identify potential biomarkers, we developed a screening immunoprecipitation-sequencing approach based on inflammatory brain glia maturation factor beta (GMFß). We discovered that a homologue of GMFß in human plasma is low molecular weight vitamin D binding protein (DBP-L). DBP-L was 8 times more abundant in plasma of BD compared to control participants. DBP-L is a product of enzymatic cleavage, transforming DBP into a macrophage-activating factor. DBP levels in participants’ plasma were validated using western blot because mono- and polyclonal ELISA did not recognize DBP-L. We found significantly increased levels of total DBP and DBP-L in BD compared to control participants. The DBP responds early to cellular damage by scavenging structural proteins and activating inflammatory cells. Our data suggest DBP and DBP-L as marker candidates of BD warranting their validation in a larger cohort of adolescent and adult MMD patients.
Ouliana Ziouzenkova (Advisor)
Barbara Gracious (Committee Member)
Martha Belury (Committee Member)
78 p.
Nutrition
vitamin D binding protein; bipolar disorder; major depressive disorder; inflammation; DBP-MAF; macrophage activating factor; inflammation; western blot; glycosylation; actin; immunoprecipitation; bait

Recommended Citations

Citations

  • Petrov, B. R. (2017). A New Role for Vitamin D Binding Protein in Bipolar Disorder [Master's thesis, Ohio State University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=osu1492642404941773

    APA Style (7th edition)

  • Petrov, Brawnie. A New Role for Vitamin D Binding Protein in Bipolar Disorder. 2017. Ohio State University, Master's thesis. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=osu1492642404941773.

    MLA Style (8th edition)

  • Petrov, Brawnie. "A New Role for Vitamin D Binding Protein in Bipolar Disorder." Master's thesis, Ohio State University, 2017. http://rave.ohiolink.edu/etdc/view?acc_num=osu1492642404941773

    Chicago Manual of Style (17th edition)

osu1492642404941773
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© 2017, some rights reserved.Creative Commons License A New Role for Vitamin D Binding Protein in Bipolar Disorder by Brawnie Rebecca Petrov is licensed under a Creative Commons Attribution-NonCommercial-NoDerivs 3.0 Unported License. Based on a work at etd.ohiolink.edu.
This open access ETD is published by The Ohio State University and OhioLINK.