Skip to Main Content
 

Global Search Box

 
 
 

ETD Abstract Container

Abstract Header

Screening for enteric coronaviruses in fecal samples of feral pigs of California, USA

Ghimire, Shristi
http://orcid.org/0000-0002-8121-9068
http://rave.ohiolink.edu/etdc/view?acc_num=osu149259749972315

Abstract Details

2017, Master of Science, Ohio State University, Comparative and Veterinary Medicine.
Enteric coronaviruses (CoVs) of pigs include transmissible gastroenteritis virus (TGEV), porcine epidemic diarrhea virus (PEDV) and porcine delta coronavirus (PDCoV). They cause significant economic losses in pig industry worldwide. In the US, PEDV emerged in 2013 and caused the death of over 7 million piglets within the first year. Feral pigs have contributed to the transmission of different viral, bacterial and parasitic infections to domestic pigs, other domestic animals and even to humans. However, their role in maintenance and transmission of porcine enteric CoVs is not well understood. In this study, fecal samples of 44 feral pigs were collected from California, USA in early-mid 2016. RNA was extracted from the fecal content supernatant and subjected to conventional reverse transcription (RT)-PCR using two different panCoV primers to detect the presence or absence of porcine enteric CoVs. Quantitative RT-PCR (RT-qPCR) using PEDV N gene and PDCoV M gene specific primers and probes were also performed for amplification of specific viral genes. DNA sequencing of PCR products from likely positive sample was also performed for further confirmation. None of the samples were clearly positive using conventional RT-PCR assays. Few samples were doubtful for PEDV or PDCoV genes and showed Ct values slightly above the detection limit. Repeated testing and/or DNA sequencing confirmed that those samples were negative for PEDV or PDCoV presence. Our results indicated that no porcine enteric CoVs were circulating in the feral pigs in California in early-mid 2016. Similar studies from other states of USA with larger sample sizes will be helpful to ascertain whether wild pigs in USA are playing a role in the maintenance and transmission of porcine enteric CoV or not.
Qiuhong Wang (Advisor)
Renukaradhya Jayamurthy Gourapura (Committee Member)
Anastasia Nickolaevna Vlasova (Committee Member)
74 p.
Epidemiology; Virology
Enteric coronaviruses; Wild pigs; Feral pigs; California; RT-PCR; RT-qPCR; PEDV; TGEV; PDCoV

Recommended Citations

Citations

  • Ghimire, S. (2017). Screening for enteric coronaviruses in fecal samples of feral pigs of California, USA [Master's thesis, Ohio State University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=osu149259749972315

    APA Style (7th edition)

  • Ghimire, Shristi. Screening for enteric coronaviruses in fecal samples of feral pigs of California, USA. 2017. Ohio State University, Master's thesis. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=osu149259749972315.

    MLA Style (8th edition)

  • Ghimire, Shristi. "Screening for enteric coronaviruses in fecal samples of feral pigs of California, USA." Master's thesis, Ohio State University, 2017. http://rave.ohiolink.edu/etdc/view?acc_num=osu149259749972315

    Chicago Manual of Style (17th edition)

osu149259749972315
558
© 2017, all rights reserved.
This open access ETD is published by The Ohio State University and OhioLINK.