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Rapid Assessment of Sugars and Organic Acids in Tomato Paste Using a Portable Mid-Infrared Spectrometer and Multivariate Analysis

Zhang, Congcong, Zhang
http://rave.ohiolink.edu/etdc/view?acc_num=osu1461245707

Abstract Details

2016, Master of Science, Ohio State University, Food Science and Technology.
Tomatoes are the second highest grown and consumed vegetables in the U.S. The majority of tomatoes are thermally processed into tomato paste, then reconstituted into various products such as tomato sauce and ketchup. As an in-between product, high-quality and consistent paste is crucial for the tomato industry. Sugars and organic acids, which are responsible for the sweetness, sourness and influence on tomato flavor, are the major factors affecting consumer acceptability and are crucial for successful processing of tomato-based products. In addition, the Vitamin C content (L-ascorbic acid and L-dehydroascorbic acid) is an attractive index for the quality of tomato product both as a source of antioxidant and Vitamin. Current analytical techniques to determine sugars and organic acids of tomato paste rely on chromatography, which is time-consuming and labor-intensive. Cutting edge infrared sensor technologies can provide a valuable window into in-process food manufacturing to permit optimization of production rate, quality and safety of tomato products. The objective of this study was to develop a rapid and robust method for simultaneous determination of sugars (glucose, fructose and total reducing sugars) and organic acids (citric acid and total Vitamin C) in tomato paste using a portable mid-infrared spectrometer combined with multivariate analysis. Tomato paste samples (n=120) were kindly provided by a major tomato processing company in California. The spectra were directly collected by a portable mid-infrared spectrometer equipped with a triple reflection diamond ATR sampling device. High-performance liquid chromatography (HPLC) was used to determine the reference levels of reducing sugars (glucose, fructose and total reducing sugars) and organic acids (citric acid and Vitamin C). Partial least square regression (PLSR) was used to develop calibration and validation models. Paste compositional ranges were glucose (6.46-13.05 g/100g), fructose (6.82-14.29 g/100g), total reducing sugars (13.28-27.01 g/100g), citric acid (2.89-5.86 g/100g) and Vitamin C (74.30-106.77 mg/100g). PLSR models showed good correlation (RCV>0.91, RVal>0.93) between the mid-infrared spectrometer predicted values and reference values, and low standard errors of cross validation (SECV) of 0.57 g/100g for glucose and 0.69 g/100g for fructose, 1.15 g/100g for total reducing sugars, 0.29g/100g for citric and 2.44 mg/100g for total Vitamin C. Portable mid-infrared spectrometer could be a revolutionary tool for in-plant assessment of the quality of tomato-based products, which would provide the tomato industry with accurate results in less time and lower cost since no reagents nor sample preparation are required.
Luis Rodriguez-Saona, Professor (Advisor)
Lynn Knipe, Associate Professor (Committee Member)
Monica Giusti, Associate Professor (Committee Member)
71 p.
Food Science
Tomato paste, mid-infrared spectrometer, PLSR, reducing sugars, organic acids

Recommended Citations

Citations

  • Zhang, Zhang, C. (2016). Rapid Assessment of Sugars and Organic Acids in Tomato Paste Using a Portable Mid-Infrared Spectrometer and Multivariate Analysis [Master's thesis, Ohio State University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=osu1461245707

    APA Style (7th edition)

  • Zhang, Zhang, Congcong. Rapid Assessment of Sugars and Organic Acids in Tomato Paste Using a Portable Mid-Infrared Spectrometer and Multivariate Analysis. 2016. Ohio State University, Master's thesis. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=osu1461245707.

    MLA Style (8th edition)

  • Zhang, Zhang, Congcong. "Rapid Assessment of Sugars and Organic Acids in Tomato Paste Using a Portable Mid-Infrared Spectrometer and Multivariate Analysis." Master's thesis, Ohio State University, 2016. http://rave.ohiolink.edu/etdc/view?acc_num=osu1461245707

    Chicago Manual of Style (17th edition)

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