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Characterization of Lab and Novel Agrobacterium Species for Development of New Tools for Plant Transformations

Marty, DeeMarie
http://rave.ohiolink.edu/etdc/view?acc_num=osu1406138595

Abstract Details

2014, Master of Science, Ohio State University, Plant Pathology.
Developing new tools that increase plant transformation efficiencies are needed for continued improvement of transgenic crop technology. Farmers are expected to plant an all-time record number of acres of soybeans in the United States in 2014. Approximately 90% of these acres will consist of genetically engineered (GE) soybeans. (www.ers.usda.gov). Most transgenic plants today are transformed using a lab modified soil-borne bacterium, Agrobacterium tumefaciens, which is known for its unique ability to transfer a piece of DNA (T-DNA) into the genome of its host plant. Removing the genes in the T-DNA region (also known as disarming) and using a vector containing gene sequences of particular interest, such as glyphosate resistance, field crops, i.e. Roundup Ready soybeans, have been developed and extensively deployed. Most Agrobacterium-mediated transformations to date have utilized a single strain of Agrobacterium called C58, which while good for transforming some crops, i.e. tobacco, can be inefficient for use in transforming recalcitrant crops, such as soybean. Currently there are many partially characterized wild-type strains of Agrobacterium spp. that have not been fully tested for use in plant transformations. In addition to these strains, soil samples from soybean fields in Ohio and nationwide, are being processed to isolate potential novel strains for further testing. From these strains a few will be selected for disarming with the intention of increasing plant transformation efficiencies.
Christopher Taylor (Advisor)
Sally Miller (Committee Member)
John Finer (Committee Member)
126 p.
Agriculture; Plant Biology; Plant Pathology; Plant Sciences
Agrobacterium, disarming, microbiology, cloning, genome sequencing

Recommended Citations

Citations

  • Marty, D. (2014). Characterization of Lab and Novel Agrobacterium Species for Development of New Tools for Plant Transformations [Master's thesis, Ohio State University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=osu1406138595

    APA Style (7th edition)

  • Marty, DeeMarie. Characterization of Lab and Novel Agrobacterium Species for Development of New Tools for Plant Transformations. 2014. Ohio State University, Master's thesis. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=osu1406138595.

    MLA Style (8th edition)

  • Marty, DeeMarie. "Characterization of Lab and Novel Agrobacterium Species for Development of New Tools for Plant Transformations." Master's thesis, Ohio State University, 2014. http://rave.ohiolink.edu/etdc/view?acc_num=osu1406138595

    Chicago Manual of Style (17th edition)

osu1406138595
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This open access ETD is published by The Ohio State University and OhioLINK.