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The role of arachidonic and docosahexaenoic acid in the alteration of hepatic fuel utilization throughout the perinatal period of the pig

Campbell, Jenny A.
http://rave.ohiolink.edu/etdc/view?acc_num=osu1233333939

Abstract Details

2009, Master of Science, Ohio State University, Animal Sciences.
A paucity of research is available on the effects of docosahexaenoic (DHA) and arachidonic (AA) acid on hepatic fuel utilization in infants. Pigs serve as suitable models for infants due to many physiological and biological similarities. To evaluate the effect of these fatty acids on partitioning fatty acids and glucose towards utilization or storage, pigs were delivered by cesarean section at 106d and 114d of gestation and umbilical catheters were placed for total parenteral nutrition (TPN). Pigs received either a control, a low polyunsaturated fatty acid (PUFA) (0.3% and 0.63% of total lipids as DHA and AA, respectively), or a high PUFA (5.5% and 12% of total lipids as DHA and AA, respectively) diet. Diets were administered based on pig weight to promote moderate weight gain. Hepatic fatty acid oxidative and glucose oxidative capacities, hepatic carnitine palmitoyltransferase (CPT) I activity and relative transcript amounts, and hepatic phoshofructokinase-1 (PFK-1) activity and relative transcript amounts were determined after 6d. Rates of fatty acid oxidation were similar across TPN regimens in both term and preterm pigs. Additionally, no differences were found between term and preterm pigs. Low PUFA administered term pigs exhibited an increase in CPT I transcript abundance (2 fold) relative to newborn pigs (P=0.031). Preterm pigs displayed increased malonyl-CoA insensitive CPT activity over term pigs in each TPN regimen (p<0.05). Preterm control and high PUFA pigs displayed a reduction in malonyl-CoA insensitive CPT activity as compared preterm low PUFA pigs (P=0.05). Rates of glucose oxidation were increased in term pigs receiving the high PUFA regimen compared to term pigs receiving the control diet (P=0.03). Additionally, term pigs receiving the low and high PUFA regimens displayed increased glucose oxidation relative to their respective preterm dietary counterparts (P<0.0001). Preterm control and high PUFA administered pigs displayed a 2-fold increase in PFK-1 transcript amounts relative to term animals (P<0.05). Term Low PUFA pigs displayed greater PFK-1 activity compared to term control pigs (P=0.03). Among term pigs all dietary treatments displayed a greater rate of glucose oxidation than fatty acid oxidation (P<0.05). Collectively, these findings suggest that fatty acid oxidation is not altered by the presence of DHA/AA in either age group regardless of changes in CPT I expression or activity. In contrast, glucose oxidation in term pigs is increased with increased concentrations of PUFA indicating an age-related PUFA effect on glucose oxidative capacity, which is not reflected in PFK-1 mRNA or activity.
Pasha Lyvers Peffer, PhD (Advisor)
Kichoon Lee, PhD (Committee Member)
Sandra Velleman, PhD (Committee Member)
104 p.
Agriculture; Animals; Livestock; Nutrition; Veterinary Services
arachidonic acid; docosahexaenoic acid; hepatic; fuel utilization; perinatal period; pig

Recommended Citations

Citations

  • Campbell, J. A. (2009). The role of arachidonic and docosahexaenoic acid in the alteration of hepatic fuel utilization throughout the perinatal period of the pig [Master's thesis, Ohio State University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=osu1233333939

    APA Style (7th edition)

  • Campbell, Jenny. The role of arachidonic and docosahexaenoic acid in the alteration of hepatic fuel utilization throughout the perinatal period of the pig. 2009. Ohio State University, Master's thesis. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=osu1233333939.

    MLA Style (8th edition)

  • Campbell, Jenny. "The role of arachidonic and docosahexaenoic acid in the alteration of hepatic fuel utilization throughout the perinatal period of the pig." Master's thesis, Ohio State University, 2009. http://rave.ohiolink.edu/etdc/view?acc_num=osu1233333939

    Chicago Manual of Style (17th edition)

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This open access ETD is published by The Ohio State University and OhioLINK.
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