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Mass Spectrometry-based Fragmentation Chemistry of Small Biological Compounds

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2021, Doctor of Philosophy (PhD), Ohio University, Chemistry and Biochemistry (Arts and Sciences).
In this dissertation, the gas-phase fragmentation chemistries of different biological compounds were characterized. We employed the tandem mass spectra of the analytes and detailed density functional theory (DFT) reaction pathway calculations to rationalize the gas-phase fragmentation mechanisms. We investigated the fragmentation characteristics of doubly protonated small peptides of different lengths and compositions (Chapter 2). We studied the fragmentation mechanism of deprotonated peptide that can offer complementary information for the most utilized protonated peptide analysis (Chapter 3). The fragmentation chemistries of different peptidoglycans which were characterized in Chapter 4. In addition, we have developed a method for facile differentiation of acidic, isomeric metabolites by attaching high proton affinity, piperidine-based chemical tags to carboxylic acid groups followed by LC-MS/MS analysis (Chapter 5).
Benjamin Bythell (Advisor)
290 p.

Recommended Citations

Citations

  • Guan, S. (2021). Mass Spectrometry-based Fragmentation Chemistry of Small Biological Compounds [Doctoral dissertation, Ohio University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=ohiou1616802495967559

    APA Style (7th edition)

  • Guan, Shanshan. Mass Spectrometry-based Fragmentation Chemistry of Small Biological Compounds. 2021. Ohio University, Doctoral dissertation. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=ohiou1616802495967559.

    MLA Style (8th edition)

  • Guan, Shanshan. "Mass Spectrometry-based Fragmentation Chemistry of Small Biological Compounds." Doctoral dissertation, Ohio University, 2021. http://rave.ohiolink.edu/etdc/view?acc_num=ohiou1616802495967559

    Chicago Manual of Style (17th edition)