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Microfluidic Device for Phenotype-Dependent Cell Agility Differentiation and Corresponding Device Sensory Implementation

Starr, Kameron D.
http://rave.ohiolink.edu/etdc/view?acc_num=ohiou1502896489271416

Abstract Details

2017, Master of Science (MS), Ohio University, Biomedical Engineering (Engineering and Technology).
The role mechanical properties play in the interconnected network of cellular control mechanisms is becoming better understood. Specifically, mechanical stiffness has been shown to be a marker capable of distinguishing between malignant and benign cancer phenotypes. Traditional techniques to measure cell stiffness share the commonality of low throughput. Microfluidic technology has been used to attain stiffness related data at a high throughput, however data collection and analysis is almost exclusively reliant on video spectroscopy. Through the use of a serial multi-constriction microfluidic device, cell ease of transit, i.e., agility, can be measured by the transit through the serial network developed herein. This measure of agility has the capability to differentiate cells based on phenotype, specifically phenotypes characteristic of the epithelial-to-mesenchymal transition, EMT, which occurs in cancer cells upon initiation of metastasis. By developing a compatible microfluidic sensor, the post processing of cell agility data has the potential to be automated and moved toward a non-video spectroscopy dependent system. These improvements push the technology of cellular mechanical property data analysis toward a faster, more convenient platform, thus allowing a better understanding of how mechanical properties correspond with biological behavior of mammalian cells.
Monica Burdick, Dr. (Advisor)
Robert Williams, Dr. (Committee Member)
Douglas Goetz, Dr. (Committee Member)
Allan Showalter, Dr. (Committee Member)
136 p.
Biomechanics; Biomedical Engineering
EMT; MET; CTC; Metastasis; Cell Agility; Agility; Microfluidic; Microfluidic Device; Migration; Sensor; Biosensor; Microfluidic Sensor; Biomechanical; MDA-MB-468; Hs 578T; Prototype; Cell Sensor; Single Cell; Single Cell Analysis; Cell Analysis; Cancer

Recommended Citations

Citations

  • Starr, K. D. (2017). Microfluidic Device for Phenotype-Dependent Cell Agility Differentiation and Corresponding Device Sensory Implementation [Master's thesis, Ohio University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=ohiou1502896489271416

    APA Style (7th edition)

  • Starr, Kameron. Microfluidic Device for Phenotype-Dependent Cell Agility Differentiation and Corresponding Device Sensory Implementation. 2017. Ohio University, Master's thesis. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=ohiou1502896489271416.

    MLA Style (8th edition)

  • Starr, Kameron. "Microfluidic Device for Phenotype-Dependent Cell Agility Differentiation and Corresponding Device Sensory Implementation." Master's thesis, Ohio University, 2017. http://rave.ohiolink.edu/etdc/view?acc_num=ohiou1502896489271416

    Chicago Manual of Style (17th edition)

ohiou1502896489271416
218
© 2017, all rights reserved.
This open access ETD is published by Ohio University and OhioLINK.