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The Effect of STAT5 on Inflammation-Related Gene Expression in Diabetic Mouse Kidneys

Shaw, Samantha J.
http://rave.ohiolink.edu/etdc/view?acc_num=ohiou1397841101

Abstract Details

2014, Master of Science (MS), Ohio University, Biological Sciences (Arts and Sciences).
Diabetic nephropathy (DN) is the leading cause of end-stage renal disease and renal failure in humans. The molecular pathways that lead to DN are not well known. This research investigates possible roles of several signal transducers and activators of transcription (STAT) proteins in this disease using a STAT5A/B knockout (SKO) mouse model. Based on previous observations of increased inflammation-related gene expression in the kidneys of diabetic SKO mice, the hypothesis of the current project was that the combination of the loss of STAT5 repression and increase of STAT3 activity escalates inflammation-related gene expression in the kidneys of diabetic SKO mice. In support of this hypothesis, an increase of IRF-1 RNA expression, reflective of the loss of STAT5 repression, was observed in the kidneys of diabetic SKO mice. Levels of phosphorylated STAT3 were also increased in the kidneys of diabetic SKO mice. These results suggest that STAT5 acts as a repressor of inflammation-related genes in DN and, in its absence, expression of these genes is no longer repressed, either due to direct loss of the STAT5 repression or due to increased STAT3 activity which could potentially increase their expression.
Karen Coschigano, PhD (Advisor)
Calvin James, PhD (Committee Member)
Ramiro Malgor, MD (Committee Member)
72 p.
Animals; Biology; Biomedical Research; Immunology; Molecular Biology
signal transducer and activator of transcription; STAT; diabetic nephropathy; inflammation; diabetes; stat5 knockout mice; mice;

Recommended Citations

Citations

  • Shaw, S. J. (2014). The Effect of STAT5 on Inflammation-Related Gene Expression in Diabetic Mouse Kidneys [Master's thesis, Ohio University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=ohiou1397841101

    APA Style (7th edition)

  • Shaw, Samantha. The Effect of STAT5 on Inflammation-Related Gene Expression in Diabetic Mouse Kidneys . 2014. Ohio University, Master's thesis. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=ohiou1397841101.

    MLA Style (8th edition)

  • Shaw, Samantha. "The Effect of STAT5 on Inflammation-Related Gene Expression in Diabetic Mouse Kidneys ." Master's thesis, Ohio University, 2014. http://rave.ohiolink.edu/etdc/view?acc_num=ohiou1397841101

    Chicago Manual of Style (17th edition)

ohiou1397841101
929
© 2014, all rights reserved.
This open access ETD is published by Ohio University and OhioLINK.