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Full text release has been delayed at the author's request until July 23, 2026

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Characterizing Genetic Bioparts to Engineer an Environmental-Isolate of Priestia Megaterium

Carter, Paul James, III
http://orcid.org/0009-0008-3083-1733
http://rave.ohiolink.edu/etdc/view?acc_num=miami1721761866958749

Abstract Details

2024, Master of Science, Miami University, Chemical, Paper and Biomedical Engineering.
Engineering microbes for sustainable bioproduction is a promising avenue to tackle problems in pollution, medicine, and energy. Traditional model organisms have received much attention due to their easily manipulated genetics and well characterized growth. However, there are a wealth of non-model microbes with attributes that are beneficial for biotechnological applications, but genetic tools must first be developed to engineer them by incorporating foreign DNA and characterizing bioparts for controlled expression. In this work, we are looking to expand the genetic toolbox for an environmental-isolate of Priestia megaterium, called SR7, that can survive under high pressures of CO2. Bioprocessing under high pressures of CO2 is expected to simultaneously create an aseptic culture environment as well as extract bioproducts as they are formed, both benefits for continuous bioproduction. An enhanced transformation method was developed, permitting bioparts characterization. We have compared promoters, origins of replication, and antibiotic resistance genes between two plasmid architectures. We identified three functional Gram-positive origins of replication for SR7. Three plasmids were verified to have enhanced recombinant protein production compared to a previously used plasmid.
Jason Boock (Advisor)
D.J. Ferguson (Committee Member)
Jason Berberich (Committee Member)
53 p.
Biology; Chemical Engineering; Microbiology; Molecular Biology
Priestia megaterium; SR7; Environmental-isolate; Nonmodel; Isobutanol; Biofuels;

Recommended Citations

Citations

  • Carter, III, P. J. (2024). Characterizing Genetic Bioparts to Engineer an Environmental-Isolate of Priestia Megaterium [Master's thesis, Miami University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=miami1721761866958749

    APA Style (7th edition)

  • Carter, III, Paul. Characterizing Genetic Bioparts to Engineer an Environmental-Isolate of Priestia Megaterium. 2024. Miami University, Master's thesis. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=miami1721761866958749.

    MLA Style (8th edition)

  • Carter, III, Paul. "Characterizing Genetic Bioparts to Engineer an Environmental-Isolate of Priestia Megaterium." Master's thesis, Miami University, 2024. http://rave.ohiolink.edu/etdc/view?acc_num=miami1721761866958749

    Chicago Manual of Style (17th edition)

miami1721761866958749
© 2024, all rights reserved.
This open access ETD is published by Miami University and OhioLINK.