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Wolfe Master's Thesis Final Draft.pdf (1.4 MB)

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Salt Adaptation for Enhanced Growth and Sucrose Production in Cyanobacteria

Wolfe, Malory Mae
http://orcid.org/0000-0003-0590-7149
http://rave.ohiolink.edu/etdc/view?acc_num=miami1626360418183729

Abstract Details

2021, Master of Science, Miami University, Microbiology.
Environmental salt concentration is an important abiotic factor indicative of environmental change and stress. As the environment changes the productivities of plants plummet thus, draining resources of the agriculture industry. Cyanobacteria are photosynthetic organisms that utilize both light and CO2 for their metabolism. Their evolutionary similarities with plants coupled with the ability to be used as a microbial feedstock make them ideal model organisms. Synthetic biology utilizes rational engineering approaches to increase the biomass and sucrose productivity of Synechococcus elongatus (Se) PCC 7942. However, these approaches are limited by a plateau effect seen in product synthesis and exhaustion of the cell. Through accelerated evolution, it is possible to induce mutations under specific stressors to drive distinct adaptations. Employing a hypermutator strain, we evolved the cells in increasing concentrations of salt and found increasing sucrose productivity following each round of evolution. Additionally, one evolved strain, Se2-192, exhibited increased salt tolerance and growth when re-exposed to salt. This project provides evidence of salt adaptation in S. elongatus PCC 7942 strains when evolved in high salt concentrations. Furthermore, the mutants produced in these studies can be used to identify adaptative mechanisms employed by the cell to deepen our understanding of metabolic plasticity.
Xin Wang, Dr. (Advisor)
Luis Actis, Dr. (Committee Member)
Donald J. Ferguson, Dr. (Committee Member)
48 p.
Microbiology
Cyanobacteria; accelerated evolution; sucrose production; salt stress

Recommended Citations

Citations

  • Wolfe, M. M. (2021). Salt Adaptation for Enhanced Growth and Sucrose Production in Cyanobacteria [Master's thesis, Miami University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=miami1626360418183729

    APA Style (7th edition)

  • Wolfe, Malory. Salt Adaptation for Enhanced Growth and Sucrose Production in Cyanobacteria. 2021. Miami University, Master's thesis. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=miami1626360418183729.

    MLA Style (8th edition)

  • Wolfe, Malory. "Salt Adaptation for Enhanced Growth and Sucrose Production in Cyanobacteria." Master's thesis, Miami University, 2021. http://rave.ohiolink.edu/etdc/view?acc_num=miami1626360418183729

    Chicago Manual of Style (17th edition)

miami1626360418183729
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© 2021, all rights reserved.
This open access ETD is published by Miami University and OhioLINK.