Skip to Main Content
 

Global Search Box

 
 
 
 

Files

File List


Thesis Jessika Hutchinson FINAL.pdf (23.47 MB)

ETD Abstract Container

Abstract Header

Elimination of microglia from the spinal cord: A model to examine plasticity following peripheral axon injury

Hutchinson, Jessika Marie
http://orcid.org/0000-0003-2110-3132
http://rave.ohiolink.edu/etdc/view?acc_num=miami1533205031089256

Abstract Details

2018, Master of Science, Miami University, Biology.
To determine the role of spinal cord microglia following a cervical sympathetic trunk transection in the periphery, mice were fed a diet containing colony-stimulating factor-1 receptor (CSF-1R) inhibitor, which eliminated spinal cord microglia. Typical neuronal and glial plasticity and changes in cytokines were compared in mice fed CSF-1R inhibitor or control diet. While neuronal plasticity was unaffected, the baseline population of oligodendrocyte precursor cells (OPCs) was increased, and following injury, typical OPC plasticity was attenuated, with increased numbers of mature oligodendrocytes (OLs) observed. The elimination of microglia did not affect baseline astrocyte morphology or glial fibrillary acidic protein (GFAP) expression, yet an increase in injury-induced GFAP response was observed. Further, without microglia, the typical injury-induced increase in interleukin-1ß expression, a proinflammatory cytokine, was blunted and a cytokine screen revealed a decrease in several additional cytokines. These alterations reveal that microglia regulate activities of OL lineage cells and astrocytes in uninjured and injured animals and contribute either directly or indirectly to the cytokine profile expressed in the spinal cord following peripheral axon injury. These findings support the need to understand the complicated cross-talk between cell populations so that such interactions might be manipulated to promote neuronal survival following injury.
Lori Isaacson (Advisor)
67 p.
Biology; Neurosciences
Microglia; astrocytes; oligodendrocyte lineage cells; glial crosstalk; PLX5622; cytokines

Recommended Citations

Citations

  • Hutchinson, J. M. (2018). Elimination of microglia from the spinal cord: A model to examine plasticity following peripheral axon injury [Master's thesis, Miami University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=miami1533205031089256

    APA Style (7th edition)

  • Hutchinson, Jessika. Elimination of microglia from the spinal cord: A model to examine plasticity following peripheral axon injury. 2018. Miami University, Master's thesis. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=miami1533205031089256.

    MLA Style (8th edition)

  • Hutchinson, Jessika. "Elimination of microglia from the spinal cord: A model to examine plasticity following peripheral axon injury." Master's thesis, Miami University, 2018. http://rave.ohiolink.edu/etdc/view?acc_num=miami1533205031089256

    Chicago Manual of Style (17th edition)

miami1533205031089256
36
© 2018, all rights reserved.
This open access ETD is published by Miami University and OhioLINK.