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Post Translational Modifications and How to Use Them

Schmitz, Benjamin P, Schmitz
http://rave.ohiolink.edu/etdc/view?acc_num=miami1524585691186704

Abstract Details

2018, Master of Science, Miami University, Cell, Molecular and Structural Biology (CMSB).
Enzymes, while incredibly complex, are limited in functionality to their twenty canonical amino acids. To shortcut this limitation, nature has evolved to utilize post translational modification (PTM) of protein structure to regulate cellular functions. Whether this be as essential cofactors or acting as regulators of enzyme activity, controlling cellular processes by PTMs are absolutely essential to the molecular-level events that coordinate and sustain life. Within a massive population of possible binding sites, enzymes responsible for PTMs manage to find their precise target through highly selective recognition sites. LplA has been shown to be a convenient tool for attaching small molecule precursors to its recognition site. The aim of this study is to modify the enzyme Lipoic acid ligase A (LplA) to effectively carve a tunnel through the backside of the enzyme. By carving a tunnel through the ligase, this work aims to broaden the substrate compatibility range of LplA for post translational modification of target proteins. Additionally in this study, crystal structures of glyceraldehyde-3-phosphate dehydrogenase (GAPDH) will be solved and analyzed for evidence of S-nitrosylation. It is the goal of this study to gain a better understanding of how this PTM regulates heme binding to GAPDH.
Richard Page (Advisor)
Michael Robinson (Committee Chair)
Yoshi Tomoyasu (Committee Member)
72 p.
Biochemistry
Protein Engineering; Structural Biology; PTMs; GAPDH; LplA

Recommended Citations

Citations

  • Schmitz, Schmitz, B. P. (2018). Post Translational Modifications and How to Use Them [Master's thesis, Miami University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=miami1524585691186704

    APA Style (7th edition)

  • Schmitz, Schmitz, Benjamin. Post Translational Modifications and How to Use Them. 2018. Miami University, Master's thesis. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=miami1524585691186704.

    MLA Style (8th edition)

  • Schmitz, Schmitz, Benjamin. "Post Translational Modifications and How to Use Them." Master's thesis, Miami University, 2018. http://rave.ohiolink.edu/etdc/view?acc_num=miami1524585691186704

    Chicago Manual of Style (17th edition)

miami1524585691186704
263
© 2018, all rights reserved.
This open access ETD is published by Miami University and OhioLINK.