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Competition between Alternative Splicing and Polyadenylation Defines the Expression of the OXT6 Gene Encoding Two Proteins Involved in mRNA Processing

Liu, Zhaoyang
http://rave.ohiolink.edu/etdc/view?acc_num=miami1281069053

Abstract Details

2010, Master of Science, Miami University, Botany.
This thesis reports a study of the interaction between messenger RNA polyadenylation and splicing in the expression of an Arabidopsis gene OXT6. This gene encodes two proteins that may involve in both polyadenylation and splicing processes. Interestingly, alternative polyadenylation or splicing of intron-2 of the gene defines the expression ratio of the two transcripts. To reveal the relationship of these processing events, a set of mutations were introduced at the splice sites and polyadenylation signals within intron-2 and transformed into the oxt6 mutant plants. The splicing and polyadenylation events were monitored by quantitative RT-PCR, revealing a competition nature of alternative polyadenylation and splicing during the OXT6 transcript processing. ChIP (chromatin immunoprecipitation) analysis performed against RNA Polymerase II in splicing mutant lines suggests that Pol II binding may affect the switch between polyadenylation and splicing during transcript processing. This work is the first of its kind that exemplifies the interplay among transcription, splicing and polyadenylation that defines a gene’s expression outcome in plants.
Qingshun Q. Li, PhD (Advisor)
Chun Liang, PhD (Committee Member)
Nicholas P. Money, PhD (Committee Member)
Molecular Biology
alternative polyadenylation; alternative splicing; mRNA processing

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Citations

  • Liu, Z. (2010). Competition between Alternative Splicing and Polyadenylation Defines the Expression of the OXT6 Gene Encoding Two Proteins Involved in mRNA Processing [Master's thesis, Miami University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=miami1281069053

    APA Style (7th edition)

  • Liu, Zhaoyang. Competition between Alternative Splicing and Polyadenylation Defines the Expression of the OXT6 Gene Encoding Two Proteins Involved in mRNA Processing. 2010. Miami University, Master's thesis. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=miami1281069053.

    MLA Style (8th edition)

  • Liu, Zhaoyang. "Competition between Alternative Splicing and Polyadenylation Defines the Expression of the OXT6 Gene Encoding Two Proteins Involved in mRNA Processing." Master's thesis, Miami University, 2010. http://rave.ohiolink.edu/etdc/view?acc_num=miami1281069053

    Chicago Manual of Style (17th edition)

miami1281069053
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© 2010, all rights reserved.
This open access ETD is published by Miami University and OhioLINK.
Release 3.2.12