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Sabyasachi Sen-MS thesis.pdf (1.33 MB)

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IMPORTANCE OF THE MAMMAL - SPECIFIC PROTEIN PHOSPHATASE ISOFORM, PPP1CC2, IN SUPPORTING SPERMATOGENESIS AND SPERM FUNCTION

Sen, Sabyasachi
http://rave.ohiolink.edu/etdc/view?acc_num=kent1416579433

Abstract Details

2014, MS, Kent State University, College of Arts and Sciences / Department of Biological Sciences.
PPP1CC1 and PPP1CC2 are alternatively spliced transcripts of PPP1CC gene. They are mostly identical, with difference only at their extreme C-termini. PPP1CC1 is ubiquitously expressed in somatic cells, while PPP1CC2 is germ cell specific. Complete deletion of PPP1CC leads to sterility due to impaired sperm morphogenesis. It has been previously documented, that fertility can be restored by optimum expression of PPP1CC2, by incorporating PPP1CC2 transgene on a PPP1CC null (-/-) background. It has also been seen that higher activity levels of PP1, renders sperm immotile but low activity levels initiate motility, as seen in caudal sperms (23). So, we wanted to determine if overexpressing PPP1CC2 in wild type (+/+) testis affected¿spermatogenesis¿ and sperm function. Mouse line was generated, having two copies of PPP1CC2 transgene on a wild type background, and was compared to wild type controls. It was found that PPP1CC2 protein as well as activity levels were highly regulated both in testis and sperm, and remained unchanged and comparable to that of wild type. Sperm were phenotypically indistinguishable from their wild type controls in terms of sperm morphology, motility and number. PPP1CC2 mRNA levels however showed a doubling in their levels in comparison to the wild type controls. Meanwhile, PPP1CC2 protein levels were found out to be drastically reduced in the mouse line, having one copy of PPP1CC1 transgene on a wild type background. There was no evidence of Protein Kinase-A mediated serine-threonine phosphorylation in these mice. Mice having one copy of PPP1CC1 transgene on a wild type background was found to be sub-fertile. Our studies suggest, perhaps, the high level of regulation of PPP1CC2 and maintenance of optimal levels of PPP1CC2 expression is crucial for maintaining normal spermatogenesis and sperm function.
Srinivasan Vijayaraghavan, Dr (Advisor)
Wen-Hai Chou, Dr (Committee Member)
Douglas Kline, Dr (Committee Member)
78 p.
Cellular Biology; Molecular Biology
PPP1CC2, PPP1CC1, PKA, Spermatogenesis, Sperm function, Regulation

Recommended Citations

Citations

  • Sen, S. (2014). IMPORTANCE OF THE MAMMAL - SPECIFIC PROTEIN PHOSPHATASE ISOFORM, PPP1CC2, IN SUPPORTING SPERMATOGENESIS AND SPERM FUNCTION [Master's thesis, Kent State University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=kent1416579433

    APA Style (7th edition)

  • Sen, Sabyasachi. IMPORTANCE OF THE MAMMAL - SPECIFIC PROTEIN PHOSPHATASE ISOFORM, PPP1CC2, IN SUPPORTING SPERMATOGENESIS AND SPERM FUNCTION . 2014. Kent State University, Master's thesis. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=kent1416579433.

    MLA Style (8th edition)

  • Sen, Sabyasachi. "IMPORTANCE OF THE MAMMAL - SPECIFIC PROTEIN PHOSPHATASE ISOFORM, PPP1CC2, IN SUPPORTING SPERMATOGENESIS AND SPERM FUNCTION ." Master's thesis, Kent State University, 2014. http://rave.ohiolink.edu/etdc/view?acc_num=kent1416579433

    Chicago Manual of Style (17th edition)

kent1416579433
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© 2014, some rights reserved.Creative Commons License IMPORTANCE OF THE MAMMAL - SPECIFIC PROTEIN PHOSPHATASE ISOFORM, PPP1CC2, IN SUPPORTING SPERMATOGENESIS AND SPERM FUNCTION by Sabyasachi Sen is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 3.0 Unported License. Based on a work at etd.ohiolink.edu.
This open access ETD is published by Kent State University and OhioLINK.
Release 3.2.12