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Full text release has been delayed at the author's request until May 06, 2025

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An Interdisciplinary Study of SARS-CoV2’s and Post-COVID-19 Syndrome: Cellular and Clinical Considerations

Singh, Aditi
http://orcid.org/0000-0002-7209-7759
http://rave.ohiolink.edu/etdc/view?acc_num=dayton1682686711930961

Abstract Details

2023, Master of Science (M.S.), University of Dayton, Interdisciplinary Studies.
The SARS-CoV2 virus was responsible for the COVID-19 Pandemic, one of the most fatal international public health emergencies experienced in the past century. SARS-CoV2 induces symptoms like increased inflammatory response, severe acute respiratory syndrome (SARS), cognitive dysfunction like brain fog, and cardiovascular defects. Prolonged or long-term infection led to the emergence of Post-COVID-19 Syndrome, or PCS. PCS is characterized by chronic cardiovascular, autoimmune, and neurological manifestations and remains understudied. Individuals with pre-existing neurological insult like those with neuroinflammatory or neurodegenerative diseases are likely more vulnerable to such PCS effects. Furthermore, individuals with pre-existing neurological conditions often have comorbidities like obesity, hypertension, hyperlipidemia, and low activity levels. However, little is understood about the molecular effects of SARS-CoV2 on neuron in both healthy and neuro-compromised individuals. Currently, many individuals experiencing PCS-related neurological symptoms require management of their symptoms even though our knowledge in this area is still limited. Therefore, this study utilized an interdisciplinary approach to better understand how SARS-CoV2 impacts both neurons at a cellular level and clinically in neurologically compromised populations such as Multiple Sclerosis (MS). This interdisciplinary approach sheds light on how translational work is being done where basic science efforts complement efforts made clinically to make connections and identify relationships between observed effects and known science. To do so, SARS-CoV2 proteins were misexpressed in the Drosophila eye and through a forward genetic screen evaluated for changes to cellular structure or function. To corroborate these findings, SARS-CoV2 proteins were also transfected into Neuro-2a cells to assess how these proteins affected cellular functioning. Furthermore, SARS-CoV2 protein structure-function analysis was also assessed in a neurodegenerative background in Drosophila. We hypothesized that SARS-CoV2 proteins may influence such backgrounds by promoting or worsening neurodegenerative effects. To better understand the clinical implications of COVID-19 on individuals with prior neurological dysfunction, a literature review was conducted to explore the effects of COVID-19 in persons with multiple sclerosis (MS). In this review symptoms were grouped by domains (e.g., cardiovascular, respiratory) that were compromised along with common interventions and methods for assessing the impacts of COVID-19 and response to treatment interventions.
Kurt Jackson (Advisor)
Mrigendra Rajput (Advisor)
108 p.
Biomedical Research; Neurology; Neurosciences; Physical Therapy; Virology
SARS-CoV2 Neurological Effects; Alzheimer's Disease SARS-CoV2 Drosophila; Multiple Sclerosis SARS-CoV2; Cell death SARS-CoV2 Neuro-2a; Cell death in N2A post SARS-CoV2 infection; Neurodegeneration SARS-CoV2; PwMS COVID-19 Neurological Manifestations; SARS-CoV2 apoptosis; SARS-CoV2 autophagy; SARS-CoV2 necrosis

Recommended Citations

Citations

  • Singh, A. (2023). An Interdisciplinary Study of SARS-CoV2’s and Post-COVID-19 Syndrome: Cellular and Clinical Considerations [Master's thesis, University of Dayton]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=dayton1682686711930961

    APA Style (7th edition)

  • Singh, Aditi. An Interdisciplinary Study of SARS-CoV2’s and Post-COVID-19 Syndrome: Cellular and Clinical Considerations. 2023. University of Dayton, Master's thesis. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=dayton1682686711930961.

    MLA Style (8th edition)

  • Singh, Aditi. "An Interdisciplinary Study of SARS-CoV2’s and Post-COVID-19 Syndrome: Cellular and Clinical Considerations." Master's thesis, University of Dayton, 2023. http://rave.ohiolink.edu/etdc/view?acc_num=dayton1682686711930961

    Chicago Manual of Style (17th edition)

dayton1682686711930961
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This open access ETD is published by University of Dayton and OhioLINK.