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Neurochemical Status and Cortical Oscillatory Activity in a Genetic Mouse Model

Klocke, Benjamin
http://rave.ohiolink.edu/etdc/view?acc_num=dayton1596121308843991

Abstract Details

2020, Master of Science (M.S.), University of Dayton, Biology.
Calcium (Ca2+) ions comprise critical second messengers for a wide variety of cellular processes, including gene expression, cell proliferation and death, and metabolism; neurons are no exception to this. Intraneuronal Ca2+ handling regulates processes such as long-term potentiation (LTP), synaptic transmission, and generation of firing patterns. Subsequently, disruptions of neuronal Ca2+ has been implicated in several neuropsychiatric and neurodegenerative disorders. A key component of the neuronal Ca2+ handling toolkit is the sarco/endoplasmic reticulum Ca2+ ATPase (SERCA) pump, specifically the SERCA2 isoform. We have recently identified a novel SERCA regulator to be expressed in the central nervous system. In the context of the current thesis, we assessed how loss of this gene may affect the neurochemical status and the cortical oscillatory activity upon genetic ablation of this gene in mice. Specifically, in the first part of the study an ex vivo neurochemical screening in distinct brain regions was conducted using high performance liquid chromatography (HPLC) with coulometric detection focusing on monoaminergic (i.e., noradrenaline, serotonin, dopamine) and aminoacidergic neurotransmission (i.e., glutamate, aspartate, γ-aminobutyric acid); in the second part of the study an electroencephalogram (EEG)-based power spectral analysis was conducted in order to assess how loss of this molecular player affects cortical oscillations in the different vigilance states in mice lacking this gene and in their wild type controls. Overall, current findings show that ablation of this gene results in sex-dependent and brain region-specific endophenotypic alterations and further provide valuable insights to understanding the role of this novel player in brain physiology and pathophysiology.
Pothitos Pitychoutis, Ph.D. (Advisor)
Karolyn Hansen, Ph.D. (Committee Member)
Amit Singh, Ph.D. (Committee Member)
62 p.
Biology; Neurobiology; Neurosciences
calcium; SERCA2; HPLC; EEG; neurochemistry; power spectral analysis; knockout;

Recommended Citations

Citations

  • Klocke, B. (2020). Neurochemical Status and Cortical Oscillatory Activity in a Genetic Mouse Model [Master's thesis, University of Dayton]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=dayton1596121308843991

    APA Style (7th edition)

  • Klocke, Benjamin. Neurochemical Status and Cortical Oscillatory Activity in a Genetic Mouse Model. 2020. University of Dayton, Master's thesis. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=dayton1596121308843991.

    MLA Style (8th edition)

  • Klocke, Benjamin. "Neurochemical Status and Cortical Oscillatory Activity in a Genetic Mouse Model." Master's thesis, University of Dayton, 2020. http://rave.ohiolink.edu/etdc/view?acc_num=dayton1596121308843991

    Chicago Manual of Style (17th edition)

dayton1596121308843991
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This open access ETD is published by University of Dayton and OhioLINK.