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Thermoresponsive Elastin-like Polypeptide Triblock for Cell Encapsulation and 3D Bioprinting Applications

Mistry, Adil
http://rave.ohiolink.edu/etdc/view?acc_num=csu1677589122731204

Abstract Details

2023, Master of Science in Chemical Engineering, Cleveland State University, Washkewicz College of Engineering.
Existing hydrogel materials used as bioinks in tissue engineering, especially bioprinting, are not engineered specifically for these applications and so have various tradeoffs or disadvantages that create limitations or incompatibilities with various cultures, equipment, or experimental conditions. Typically, naturally derived hydrogels demonstrate good biocompatibility but with poor rheological properties, whereas synthetically produced polymers typically demonstrate excellent printability at the cost of biocompatibility. To bridge these material needs, a triblock copolymer elastin-like polypeptide hydrogel containing RGDS cell binding domains to enhance biocompatibility was designed, produced, and purified through recombinant expression in Escherichia coli. This material was characterized through DLS measurements to understand purification characteristics and the micelle geometry used to estimate a minimum gelation concentration of the novel construct is estimated to be between 10 mM and 0.6 mM (0.3 g/mL and 0.018 g/mL). Further work will involve characterizing the rheological properties of the networked micelle hydrogel.
Nolan Holland, Ph.D. (Committee Chair)
Chelsea Monty-Bromer, Ph.D. (Committee Member)
Metin Uz, Ph.D. (Committee Member)
102 p.
Biomedical Engineering; Molecular Biology
Tissue Engineering; Hydrogels; Elastin-like polypeptides; ELPs; Biomaterials

Recommended Citations

Citations

  • Mistry, A. (2023). Thermoresponsive Elastin-like Polypeptide Triblock for Cell Encapsulation and 3D Bioprinting Applications [Master's thesis, Cleveland State University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=csu1677589122731204

    APA Style (7th edition)

  • Mistry, Adil. Thermoresponsive Elastin-like Polypeptide Triblock for Cell Encapsulation and 3D Bioprinting Applications. 2023. Cleveland State University, Master's thesis. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=csu1677589122731204.

    MLA Style (8th edition)

  • Mistry, Adil. "Thermoresponsive Elastin-like Polypeptide Triblock for Cell Encapsulation and 3D Bioprinting Applications." Master's thesis, Cleveland State University, 2023. http://rave.ohiolink.edu/etdc/view?acc_num=csu1677589122731204

    Chicago Manual of Style (17th edition)

csu1677589122731204
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© 2023, all rights reserved.
This open access ETD is published by Cleveland State University and OhioLINK.