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Life Cycle and Morphological Characterization of Colpodella sp. (ATCC 50594) in Hay Medium

Getty, Troy A
http://rave.ohiolink.edu/etdc/view?acc_num=csu1611795646162548

Abstract Details

2020, Master of Science in Biology, Cleveland State University, College of Sciences and Health Professions.
Colpodella species are free living biflagellated protists that prey on algae and bodonids in a process known as myzocytosis. Colpodella species are phylogenetically closely related to Apicomplexa. Cyst and trophozoite stages have been identified in Colpodella species, however the mechanisms of encystation and excystation are unknown. The timing and transition of the life cycle stages are unknown. In the present study I investigated the life cycle of Colpodella sp. (ATCC 50594). Colpodella sp. were grown in diprotist cultures in tissue culture flasks containing Hay medium bacterized with Enterobacter aerogenes and containing Bodo caudatus as prey. I wanted to know the timing and duration of the life cycle and the transition stages of Colpodella sp. Six time course experiments were performed to determine the duration of the life cycle stages, to investigate the transition of stages within the life cycle, and to identify the predominant cyst stage in resting cultures. In all time course experiments cells were formalin-fixed and stained with Sam-Yellowe’s trichrome for visualization by light microscopy. Confocal and differential interference contrast (DIC) microscopy was performed to determine cross reactivity of Plasmodium species and Toxoplasma gondii specific antibodies against Colpodella sp. proteins and to identify cell morphology, respectively. Transmission electron microscopy (TEM) was performed to visualize the ultrastructure of Colpodella sp. Data obtained shows the duration of Colpodella sp. life cycle is thirty-six hours. Colpodella sp. were most active between 20-28 hours. Trophozoites actively swimming, feeding, forming transient cysts, and excysting were observed. Bodo caudatus excysted and encysted earlier in the culture. Previously undocumented life cycle stages identified include precyst stages, multinucleate cysts, egress of trophozoites with incomplete cytokinesis, and predominant single nuclei mature resting cysts. Multinucleate cysts of Colpodella sp. are reminiscent of Apicomplexan schizonts. Immunofluorescence confirmed reactivity between Plasmodium rhoptry, microneme and food vacuole specific antibodies as well as T. gondii inner membrane complex (IMC) antibodies with Colpodella sp. proteins. Both DIC and TEM microscopy identified asynchronous and asymmetric mitosis in Colpodella sp. cysts. Knowledge of the life cycle and stages of Colpodella sp. will allow investigators to perform studies in cell biology and biochemistry of Colpodella species.
Tobili Sam-Yellowe, Ph.D. (Advisor)
Girish Shukla, Ph.D. (Committee Member)
Michael Walton , Ph.D. (Committee Member)
120 p.
Microbiology; Parasitology

Recommended Citations

Citations

  • Getty, T. A. (2020). Life Cycle and Morphological Characterization of Colpodella sp. (ATCC 50594) in Hay Medium [Master's thesis, Cleveland State University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=csu1611795646162548

    APA Style (7th edition)

  • Getty, Troy. Life Cycle and Morphological Characterization of Colpodella sp. (ATCC 50594) in Hay Medium. 2020. Cleveland State University, Master's thesis. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=csu1611795646162548.

    MLA Style (8th edition)

  • Getty, Troy. "Life Cycle and Morphological Characterization of Colpodella sp. (ATCC 50594) in Hay Medium." Master's thesis, Cleveland State University, 2020. http://rave.ohiolink.edu/etdc/view?acc_num=csu1611795646162548

    Chicago Manual of Style (17th edition)

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