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An Insight into GAIT Complex Mediated Translational Silencing

Kapasi, Purvi
http://rave.ohiolink.edu/etdc/view?acc_num=csu1232567504

Abstract Details

2008, Master of Science in Biology, Cleveland State University, College of Science.
Transcript-specific translational control restricts macrophage inflammatory gene expression. The pero-inflammatory cytokine IFN-γ induces the phosphorylation of human ribosomal protein L13a and its subsequent release from 60S ribosome. L13a is a component of the interferon-gamma-activated inhibitor of translation (GAIT). The GAIT complex binds a defined element in the 3'-untranslated region (UTR) of ceruloplasmin (Cp) mRNA and causes delayed silencing of translation. In this research, we elucidate the molecular mechanism underlying L13a translational silencing activity. L13a mediates translational silencing particularly, when driven by internal ribosome entry sites (IRESs) that requires the initiation factor eIF4G, but is resistant to silencing when driven by eIF4F- independent IRESs. This demonstrates a critical role of the scaffold protein eIF4G. Global inhibition of protein synthesis by targeting eIF4G is well appreciated in virus infection and apoptosis; however interaction of L13a with eIF4G blocks the 43S complex recruitment showing a unique role of eIF4G in gene specific translational silencing.
Barsanjit Mazumder, PhD (Advisor)
Anton Komar, PhD (Committee Member)
Crystal Weyman, PhD (Committee Member)
49 p.
Cellular Biology; Molecular Biology
Transcript-specific transaltional control; L13a; GAIT; Ceruloplasmin; eIF4G

Recommended Citations

Citations

  • Kapasi, P. (2008). An Insight into GAIT Complex Mediated Translational Silencing [Master's thesis, Cleveland State University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=csu1232567504

    APA Style (7th edition)

  • Kapasi, Purvi. An Insight into GAIT Complex Mediated Translational Silencing. 2008. Cleveland State University, Master's thesis. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=csu1232567504.

    MLA Style (8th edition)

  • Kapasi, Purvi. "An Insight into GAIT Complex Mediated Translational Silencing." Master's thesis, Cleveland State University, 2008. http://rave.ohiolink.edu/etdc/view?acc_num=csu1232567504

    Chicago Manual of Style (17th edition)

csu1232567504
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© 2008, all rights reserved.
This open access ETD is published by Cleveland State University and OhioLINK.