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Royce Duda MS Thesis.pdf (2.21 MB)

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Genetic Manipulation and Culturing of Azotobacter vinelandii for the Production of Nitrogenase for Use in Protein-Engineered Electrochemical Systems

Duda, Royce D
http://rave.ohiolink.edu/etdc/view?acc_num=case1530282333596752

Abstract Details

2018, Master of Sciences, Case Western Reserve University, Chemical Engineering.
The industrial production of ammonia is a vital but energy intensive process. Promising alternatives to the costly Haber-Bosch process include the utilization of the enzyme nitrogenase in protein-engineered electrochemical systems. Preparation of pure molybdenum-dependent nitrogenase from the bacteria Azotobacter vinelandii is reported in this thesis. An attempt to incorporate the CRISPR-Cas9 system in A. vinelandii to streamline the process of genetic modification in A. vinelandii is also reported. Specifically, a technical approach was created to test the suitability of the pCRISPR-pCas9 system in A. vinelandii, and initial results are reported that provide evidence that the plasmid pCRISPR is suitable for replication within A. vinelandii. A curriculum featuring molecular cloning in Escherichia coli was developed for the Widening Opportunities for Women in Science Program, and survey results from this program were analyzed to reveal that it was successful in heightening female high school students’ awareness and interest in chemical engineering.
Julie Renner (Advisor)
Harihara Baskaran (Committee Member)
Heidi Martin (Committee Member)
120 p.
Chemical Engineering
Azotobacter vinelandii; Nitrogenase; Nitrogen Fixation; CRISPR; Cas9; Genetic Modification; Protein; Purification; Ammonia; MoFe

Recommended Citations

Citations

  • Duda, R. D. (2018). Genetic Manipulation and Culturing of Azotobacter vinelandii for the Production of Nitrogenase for Use in Protein-Engineered Electrochemical Systems [Master's thesis, Case Western Reserve University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=case1530282333596752

    APA Style (7th edition)

  • Duda, Royce. Genetic Manipulation and Culturing of Azotobacter vinelandii for the Production of Nitrogenase for Use in Protein-Engineered Electrochemical Systems. 2018. Case Western Reserve University, Master's thesis. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=case1530282333596752.

    MLA Style (8th edition)

  • Duda, Royce. "Genetic Manipulation and Culturing of Azotobacter vinelandii for the Production of Nitrogenase for Use in Protein-Engineered Electrochemical Systems." Master's thesis, Case Western Reserve University, 2018. http://rave.ohiolink.edu/etdc/view?acc_num=case1530282333596752

    Chicago Manual of Style (17th edition)

case1530282333596752
821
© 2018, all rights reserved.
This open access ETD is published by Case Western Reserve University School of Graduate Studies and OhioLINK.