Skip to Main Content
 

Global Search Box

 
 
 
 

Files

File List


2013 Sukhdeo PhD Thesis.pdf (60.54 MB)

ETD Abstract Container

Abstract Header

Defining immunophenotypic signatures of stem cells

Sukhdeo, Kumar
http://rave.ohiolink.edu/etdc/view?acc_num=case1373038255

Abstract Details

2013, Doctor of Philosophy, Case Western Reserve University, Pathology.
Advancement of stem cell biology is critically dependent on improved isolation and characterization of stem cells. This thesis describes a) the development of two novel screening assays for stem cells and b) characterizes the expression of the stem cell marker Lgr5 in two anatomically distinct tissue compartments. Whole-animal lineage tracing provides global in situ expression analysis, which can be used to evaluate stem cell proliferation, trophism, niche structures, and anatomical spatial relationships. This method accelerated the detection of Lgr5 in multiple tissues, including the liver and central nervous system. These expression patterns are impactful for regenerative medicine efforts aimed at isolating stem/progenitor cells. In addition, the data contained herein refine the understanding of the Wnt pathway in stem and non-stem cells as they exist within signaling gradients and different physiological states. Multiplex high-throughput flow cytometry is another technique that can be used to rapidly identify comprehensive surface antigen signatures of normal and cancer cells. The methodology developed in this thesis doubles potential coverage of immunophenotypes using reagents directly translatable into clinical application for enhancing cellular detection and development of targeted therapies. Since stem cells relate to the biology of homeostasis, regeneration, and cancer, the works completed in this thesis advance our medical knowledge and capabilities.
Jeremy Rich, MD (Advisor)
Alan Levine, PhD (Committee Chair)
Anita Hjelmeland, PhD (Committee Member)
Alex Huang, MD, PhD (Committee Member)
Sanford Markowitz, MD, PhD (Committee Member)
Clive Hamlin, PhD (Committee Member)
168 p.
Biology; Biomedical Research; Cellular Biology; Pathology
Lgr5, stem cells; cancer stem cells; colon cancer; retina, amacrine cells; liver; progenitor cells

Recommended Citations

Citations

  • Sukhdeo, K. (2013). Defining immunophenotypic signatures of stem cells [Doctoral dissertation, Case Western Reserve University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=case1373038255

    APA Style (7th edition)

  • Sukhdeo, Kumar. Defining immunophenotypic signatures of stem cells. 2013. Case Western Reserve University, Doctoral dissertation. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=case1373038255.

    MLA Style (8th edition)

  • Sukhdeo, Kumar. "Defining immunophenotypic signatures of stem cells." Doctoral dissertation, Case Western Reserve University, 2013. http://rave.ohiolink.edu/etdc/view?acc_num=case1373038255

    Chicago Manual of Style (17th edition)

case1373038255
273
© 2013, all rights reserved.
This open access ETD is published by Case Western Reserve University School of Graduate Studies and OhioLINK.