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Characterization of Pseudomonas Strains Isolated from Lake Superior that Inhibit Cystic Fibrosis Derived Pathogens 

Lenoy, Tyler
http://rave.ohiolink.edu/etdc/view?acc_num=bgsu1724346983432647

Abstract Details

2024, Master of Science (MS), Bowling Green State University, Biological Sciences.
The overuse and misuse of antibiotics has led to a global crisis in which most bacterial pathogens have evolved resistance to the available drugs used for treatments. As a result, antibiotic resistant bacterial infections are expected to cause 10 million deaths per year by 2050, passing cancer as the leading cause of mortality. Complicating the issue is that large pharmaceutical companies have stopped antibiotic discovery and development due to marginal profits, so very few new drugs are available for life-threatening infections. From previous studies in our lab, we have shown that environmental isolates of Pseudomonas strains can inhibit cystic fibrosis (CF) derived clinical pathogens, including Pseudomonas aeruginosa and members of Achromobacter and Stenotrophomonas species. This study is part of a larger project that aims to determine if certain water habitats select for antibiotic producing bacteria. Here, 271 environmental Pseudomonas strains were isolated from Lake Superior and tested for their ability to inhibit a CF-patient derived panel of 25 multidrug-resistant pathogens, including nine Burkholderia species, nine P. aeruginosa, and seven Staphylococcus aureus; also tested were five strains of Aeromonas fish pathogens. Using an antagonistic plate assay, 8,130 individual competition assays were performed, and 515 instances of competitive inhibition were observed among the pathogens. From these events, 59 were against P. aeruginosa, 66 were against Burkholderia, 273 were against S. aureus, and 53 were against Aeromonas. To identify genes involved in antagonistic activity, transposon mutagenesis was performed with environmental antagonistic Pseudomonas strains TL1E8 and TL2A8 and loss of killing mutants were identified. TL1E8 was found to inhibit strains of Pseudomonas aeruginosa, Burkholderia, and Staphylococcus aureus. Transposon mutated genes identified included sulfite reductase flavoprotein, methyl-accepting chemotaxis protein, and diguanylate cyclase-like protein. TL2A8 was found to inhibit strains of Burkholderia, Staphylococcus aureus, and Aeromonas. Transposon mutated genes identified in TL2A8 included citrate synthase and a two-component system sensor histidine kinase. Bioinformatic analyses are being pursued to predict the antagonistic products. These results suggest that environmental Pseudomonas strains from Lake Superior were active at inhibiting multidrug-resistant pathogens and may be a source of novel antibiotic discovery.
Hans Wildschutte, Ph.D. (Committee Chair)
Raymond Larsen, Ph.D. (Other)
Daniel Wiegmann, Ph.D. (Other)
47 p.
Biology; Microbiology
Pseudomonas; Antibiotics; Transposon Mutagenesis;

Recommended Citations

Citations

  • Lenoy, T. (2024). Characterization of Pseudomonas Strains Isolated from Lake Superior that Inhibit Cystic Fibrosis Derived Pathogens  [Master's thesis, Bowling Green State University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=bgsu1724346983432647

    APA Style (7th edition)

  • Lenoy, Tyler. Characterization of Pseudomonas Strains Isolated from Lake Superior that Inhibit Cystic Fibrosis Derived Pathogens  . 2024. Bowling Green State University, Master's thesis. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=bgsu1724346983432647.

    MLA Style (8th edition)

  • Lenoy, Tyler. "Characterization of Pseudomonas Strains Isolated from Lake Superior that Inhibit Cystic Fibrosis Derived Pathogens  ." Master's thesis, Bowling Green State University, 2024. http://rave.ohiolink.edu/etdc/view?acc_num=bgsu1724346983432647

    Chicago Manual of Style (17th edition)

bgsu1724346983432647
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© 2024, all rights reserved.
This open access ETD is published by Bowling Green State University and OhioLINK.