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The Behavioral Effects of Early Morphine Exposure in Drosophila melanogaster

Pudasaini, Pratikshya
http://rave.ohiolink.edu/etdc/view?acc_num=bgsu1641387990687365

Abstract Details

2022, Master of Science (MS), Bowling Green State University, Biological Sciences.
The medical use of opioids and prescriptions is a major source that causes drug dependency in people raising concern worldwide. It is a major health crisis causing thousands of deaths every year. Morphine, an opioid, is used as anesthetics in clinics worldwide and is highly overused among different age groups. Prescribed opioid has become a major source of drug availability in the market reaching mostly young people. Adolescents are more prone to morphine overdose because of the euphoria that the drug provides causing gradual development of drug dependency and causing higher negative impacts than in adults. Our study focused on the effects of early morphine exposure on motor behavior at different time periods of life using Drosophila melanogaster which is a suitable animal model due to its small size and measurable behaviors [1]. We hypothesized that rearing fly larva by exposure to morphine shows motor behavioral abnormalities that progress to later stages of life. We compared three morphine exposure levels, 0(control), 0.025 mg/ml and 0.25 mg/ml. Fly larvae were reared with morphine and the locomotion and feeding behavior were observed in the late second or early third instar. The feeding behavior of male larva exposed to low and high drug doses differed significantly from the control group whereas locomotion was not affected. Behavioral assays were also performed on adult flies to observe the prolonged effects of morphine exposure. Startled-Induced Negative Geotaxis (SING) assay was used to observe the climbing behavior of adult flies that differed significantly between the control and low and high-dose females. Drug-treated flies showed a climbing preference in this assay. For further observation of motor behavior on adult flies, righting reflex assay (to observe the ability of flies to correct their position) and flight assay (to observe the ability of flies in maintaining steady elevation) were also run which showed no significant difference among any groups. Our results show that maintaining a balanced body position and elevation is slightly but not significantly affected by morphine exposure. Taken together, these findings suggest that early morphine exposure has a behavioral effect on different stages of life.
Larsen Raymond A, Ph.D. (Committee Member)
Huber Robert, Ph.D. (Committee Member)
Moira J van Staaden, Ph.D. (Advisor)
43 p.
Behavioral Sciences
Opioid; morphine; Drosophila melanogaster; behavior

Recommended Citations

Citations

  • Pudasaini, P. (2022). The Behavioral Effects of Early Morphine Exposure in Drosophila melanogaster [Master's thesis, Bowling Green State University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=bgsu1641387990687365

    APA Style (7th edition)

  • Pudasaini, Pratikshya. The Behavioral Effects of Early Morphine Exposure in Drosophila melanogaster. 2022. Bowling Green State University, Master's thesis. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=bgsu1641387990687365.

    MLA Style (8th edition)

  • Pudasaini, Pratikshya. "The Behavioral Effects of Early Morphine Exposure in Drosophila melanogaster." Master's thesis, Bowling Green State University, 2022. http://rave.ohiolink.edu/etdc/view?acc_num=bgsu1641387990687365

    Chicago Manual of Style (17th edition)

bgsu1641387990687365
214
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This open access ETD is published by Bowling Green State University and OhioLINK.
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