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Ph.D. Dissertation - Alicja Marlowe.pdf (8.25 MB)

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Expression of Selected Cadherins in Adult Zebrafish Visual System and Regenerating Retina, and Microarray Analysis of Gene Expression in Protocadherin-17 Morphants

Marlowe, Alicja
http://rave.ohiolink.edu/etdc/view?acc_num=akron1658589091234495

Abstract Details

2022, Doctor of Philosophy, University of Akron, Integrated Bioscience.
Cadherins are cell-adhesion molecules that play important roles in animal development, maintenance and/or regeneration of adult animal tissues. In order to understand cadherins’ functions in adult vertebrate visual structures, one must study their distribution in those structures. First, I examined expression of cadherin-6, cadherin-7, protocadherin-17 and protocadherin-19 in the visual structures of normal adult zebrafish using RNA in situ hybridization, followed by studying expression of two Krüppel-like transcription factors (klf6a and klf7), that are known markers for regenerating adult zebrafish retinas and optic nerves, in normal adult zebrafish brain, normal and regenerating adult zebrafish retinas. Then, I investigated expression of these cadherins in regenerating adult zebrafish retinas using both RNA in situ hybridization and quantitative PCR. Finally, as the first step in elucidating molecular mechanisms underlying protocadherin-17 (one of the cadherins that I studied) function in zebrafish visual system development, I used DNA microarray analysis to study gene expression of zebrafish embryos with their protocadherin-17 expression blocked by morpholino antisense oligonucleotides (these embryos are called protocadherin- 17 morphants). The major findings include: 1) cadherin-6, cadherin-7, protocadherin-17 and protocadherin-19 were differently expressed in the retina and major visual structures of normal adult zebrafish brain. 2) klf6a and klf7 showed similar expression patterns in most visual structures in the adult fish brain, and in regenerating retinas, but klf6a appeared to be a superior regeneration marker based on RNA in situ hybridization. 3) These four cadherin molecules showed distinct expression patterns in the regenerating zebrafish retinas. 4) Several genes involved in vision and/or visual development were significantly downregulated in the protocadherin-17 morphants compared to control embryos. My results suggest that these cadherins play differential roles in the maintenance and regeneration of the adult zebrafish visual system, and protocadherin-17 may function in the zebrafish visual system development via affecting expression of these genes involved in vision and/or visual development.
Qin Liu (Advisor)
Richard Londraville (Committee Member)
Rolando Ramirez (Committee Member)
Brian Bagatto (Committee Member)
Zhong-Hui Duan (Committee Member)
337 p.
Anatomy and Physiology; Bioinformatics; Biology; Developmental Biology; Molecular Biology
molecular biology; neurobiology; anatomy; gene expression; microarray; cadherin; protocadherin; transcription factor; zebrafish; visual system; retina; regeneration; CNS; development; computational biology

Recommended Citations

Citations

  • Marlowe, A. (2022). Expression of Selected Cadherins in Adult Zebrafish Visual System and Regenerating Retina, and Microarray Analysis of Gene Expression in Protocadherin-17 Morphants [Doctoral dissertation, University of Akron]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=akron1658589091234495

    APA Style (7th edition)

  • Marlowe, Alicja. Expression of Selected Cadherins in Adult Zebrafish Visual System and Regenerating Retina, and Microarray Analysis of Gene Expression in Protocadherin-17 Morphants. 2022. University of Akron, Doctoral dissertation. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=akron1658589091234495.

    MLA Style (8th edition)

  • Marlowe, Alicja. "Expression of Selected Cadherins in Adult Zebrafish Visual System and Regenerating Retina, and Microarray Analysis of Gene Expression in Protocadherin-17 Morphants." Doctoral dissertation, University of Akron, 2022. http://rave.ohiolink.edu/etdc/view?acc_num=akron1658589091234495

    Chicago Manual of Style (17th edition)

akron1658589091234495
420
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