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The Effect of Mesenchymal Stromal Cells, Platelet-Rich Plasma, and Collagen on Rat Achilles Tendon Repair

Juzbasich, Dragan
http://rave.ohiolink.edu/etdc/view?acc_num=ysu1639484732168658

Abstract Details

2021, Master of Science in Biological Sciences, Youngstown State University, Department of Biological Sciences and Chemistry.
The calcaneal (Achilles) tendon is capable of handling tremendous tensile loads during locomotion. However, cases of Achilles tendon ruptures have increased in recent years, requiring long healing times. Repaired tendons are more prone to re-rupture after healing, which may negatively impact patient quality of life. Thus, there exists a need for new methods of treatment aimed to improve and accelerate tendon healing. We studied the effect a combination of collagen, platelet-rich plasma (PRP), and mesenchymal stromal cells (MSC) on healing a complete Achilles tendon rupture in a Lewis rat model. The PRP was produced from rat blood collected during exsanguination procedures. MSCs from rat bone marrow met the criteria to be considered stem cells in a rat model, as they were seen to be plastic adherent and capable of tri-lineage differentiation. Rupture was surgically simulated by a full-thickness transection of the tendon, followed by surgical repair. All treatments included a strip of CollaTapeTM wrapped around the repair, acting as a vehicle for the biologics prior to closure of the wound. A single, 100µL subcutaneous injection of MSCs, PRP, or both were administered adjacent to the incision and assigned 1- or 2-week recovery periods before harvesting the operated and unoperated tendons. We observed promising trends which show an increase in gene expression activity in the treated tendons and differences in the expression of Col1a1 and Col3a1 which align with our predicted response to the treatments. However, due to contamination of the GAPDH RT-PCR results, the collagen analysis results remain inconclusive. The biomechanical properties of the tendons were determined using force-extension analysis. When normalized as a percent of the unoperated tendon, a significant improvement was seen in the strain at failure and in ultimate tensile strength after only one week of recovery in the rats who received any biological treatments used in this study, when compared to a surgical control. Compared to the vehicle control, using all three biologics together shows a significant improvement over adding just MSCs or PRP alone. These results suggest that this treatment yields an accelerated healing response and may reduce occurrence of rerupture after healing.
Diana Fagan, PhD (Advisor)
Gary Walker, PhD (Committee Member)
Carmen Panaitof, PhD (Committee Member)
189 p.
Biology; Biomechanics; Biomedical Research; Physiology; Surgery
Achilles Tendon; Mesenchymal Stromal/Stem Cells; Platelet-Rich Plasma; Type I Collagen Scaffold; Rat Model; Surgical Repair; Stem Cell Therapy; Biological Treatment; Wound Healing; Biomechanical Properties; Gene Expression

Recommended Citations

Citations

  • Juzbasich, D. (2021). The Effect of Mesenchymal Stromal Cells, Platelet-Rich Plasma, and Collagen on Rat Achilles Tendon Repair [Master's thesis, Youngstown State University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=ysu1639484732168658

    APA Style (7th edition)

  • Juzbasich, Dragan. The Effect of Mesenchymal Stromal Cells, Platelet-Rich Plasma, and Collagen on Rat Achilles Tendon Repair. 2021. Youngstown State University, Master's thesis. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=ysu1639484732168658.

    MLA Style (8th edition)

  • Juzbasich, Dragan. "The Effect of Mesenchymal Stromal Cells, Platelet-Rich Plasma, and Collagen on Rat Achilles Tendon Repair." Master's thesis, Youngstown State University, 2021. http://rave.ohiolink.edu/etdc/view?acc_num=ysu1639484732168658

    Chicago Manual of Style (17th edition)

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This open access ETD is published by Youngstown State University and OhioLINK.
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