Display Full Text | Download Full Text
1.53 MB PDF file

Degree

Master of Science (MS), Wright State University, Chemistry, 2010.

Abstract

Modified HPEI was used to determine if the LCST could be controlled by varying multiple variables, including: percent modification, pH, salt solution concentration, and weight percent. Two modifications were done with butylene oxide (Butox) and isobutyryl amide (IBAm). Specifically, 100% (HPEI-Butox1.00)butoxylated and 10% (HPEI-IBAm0.10), 54% (HPEI-IBAm0.54), and 70% (HPEI-IBAm0.70) amidated samples were synthesized. Additionally, HPEI-IBAm0.54 was further modified with hydroxyl ethyl groups (HPEI-IBAm0.54-Eox0.46) and ethyl groups (HPEI-IBAm0.54-Et0.44). The LCSTs of all amidated systems were below body temperature, 37 °C, at pH = 7.4. This indicates an ability of these systems to be used as a vector for gene delivery. A PBS salt solution lowered the LCST, while weight percent had a non linear effect on the LCST. Transfection data on the 54% amidated HPEI system showed transfection efficiency equivalent to control transfectant, LT1. These data suggest a strong potential for using modified HPEI systems as nonviral gene vectors.

Subject Headings

Biochemistry; Cellular biology; Chemistry

Keywords

gene delivery; polyethylenimine; polymers

Committee / Advisors

Eric Fossum, PhD (Advisor)
Kenneth Turnbull, PhD (Committee Member)
Pavel Ioana, PhD (Committee Member)

Pages

52p.

Document number: wright1284664818
Permalink: http://rave.ohiolink.edu/etdc/view?acc_num=wright1284664818

This ETD has been downloaded 403 times (through March 2013)