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Degree

Master of Science, Miami University, Physics, 2012.

Abstract

A hydrostatic pressure perfusion system with two closed fluid reservoirs has been constructed and characterized. The system utilizes two stainless steel pressure generators connected to gearheads. Stepper motors are connected to the gearheads and are used to provide constant fluid flow throughout the system. We communicate with both motors through a hub, such that we can monitor the internal pressure of the system with a pressure gauge. Fluorescence has been used to detect changes in emission spectra between the different fluids in the reservoirs. We have also demonstrated that we can trap Saccharomyces cerevisiae, or baker's yeast, in a quartz capillary tube and detect spectral changes while monitoring the internal pressure of the system.

Subject Headings

Biophysics; Physics

Keywords

hydrostatic pressure; perfusion system

Committee / Advisors

Paul Urayama, PhD (Advisor)
Michael Pechan, PhD (Committee Member)
Herbert Jaeger, PhD (Committee Member)

Document number: miami1343970397
Permalink: http://rave.ohiolink.edu/etdc/view?acc_num=miami1343970397

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