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Evolution of Group I Introns in the Nuclear Ribosomal RNA Genes of Dothideomycetes

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Degree
Master of Science (MS), Bowling Green State University, Biological Sciences, .
Abstract
Group I introns, as self-splicing ribozymes, have been very important in the evolution of fungi. In this study, a set of group I introns have been described within the nuclear small and large subunit rRNAs of several fungi. DNA extraction, ribosomal DNA amplification, sequencing, secondary structure analysis and phylogenetic analysis were performed, which led to the conclusion that the evolutionary history of group I introns has been processed as multiple insertions over a prolonged time by horizontal transmission, followed by the loss of the ability to insert into new loci, followed by an extended time of vertical inheritance, including reduction in size and loss of secondary structure segments, followed by the complete loss of group I introns.
Subject Headings
Biology
Keywords
Group I intron; Secondary Structure; Phylogenetic tree
Committee / Advisors
Scott Rogers (Advisor)
Paul Morris (Committee Member)
Vipaporn Phuntumart (Committee Member)
Pages
40p.

Document number: bgsu1288376350
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