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Degree

Master of Science, University of Akron, Biology, 2005.

Abstract

Characterizing molecular signaling in lipid metabolism traditionally has not been investigated with an integrated global mechanism approach. The peroxisome proliferated-activated receptors (PPARs) are a family of transcription factors that in response to lipids orchestrate metabolism. It was hypothesized that a high-calorie diet in zebrafish would up-regulate activity of PPARs. Proteomics identified discrete focused bands with greater than a 200% difference between the high-calorie and low-calorie diet treatment groups. Protein bands were subsequently submitted for identification with mass spectrometry. A lower total number of discrete proteins were expressed in the high-calorie group, which can be interpreted as part of a general stress response. Specific proteins up-regulated indicative of stress were prohibitin, glutamate oxaloacetate transaminase, and glyoxylase II. Down-regulated proteins indicative of stress induced retarded growth included cytokeratin, brain type fatty acid binding protein, profilin 2, and histone. Other proteins exhibited expression patterns that did not fit into a predictable stress response.

Keywords

Proteomics; Diet; Zebrafish; Liver; Calorie availability; Stress response

Advisor

Richard R. Londraville

Pages

54p.

Document number: akron1129389691
Permalink: http://rave.ohiolink.edu/etdc/view?acc_num=akron1129389691

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